Mouse Model for Cerebral Hemorrhage (CH) Mus musculus (Mouse) Disease model

Hemorrhage, Intracerebral Hemorrhage

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Overview
Properties
  • Prototype SpeciesHuman
  • Sourceinduced by Collagenase
  • Model Animal StrainsC57BL/6 Mice(SPF), healthy, male, age: 6~8 weeks, body weight:22g~25g.
  • Modeling Grouping1.Randomly divided into six group: Control group, Model group, Positive drug group and Tested drug group.
  • Modeling Period1w
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  • Mouse Model for Cerebral Hemorrhage (CH) Packages (Simulation)
  • Mouse Model for Cerebral Hemorrhage (CH) Packages (Simulation)
  • DSI836Mu01.jpg Fig.Tunel staining of Brain
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Modeling Method

The mice were anesthetized by intrabitoneal injection. 1uL of 0.1mg/mL type IV collagenase solution was injected through brain stereolocalization (the former fontanel site was at zero, 2.2mm to the left, 1.0mm forward, and 2.7mm into the needle). After the injection, the needle was kept in situ for 10 minutes to prevent collagenase reflux, and then the needle was slowly withdrawn.The body temperature of mice was maintained at 37℃ during the whole experiment.
ICH model and SHAM group were prepared, except for no collagenase injection, other operations were the same.(Note that collagenase should be placed on an ice pack to keep the temperature low)

Model evaluation

Neurological function detection and assessment:
The 5-point system of Longa and Bederson was used to score the animals 24h after waking up from anesthesia. The higher the score, the more serious behavior disorder of the animals.
0 points: no symptoms of nerve damage
1 point: Can't fully extend the opposite forepaw
2 points: Turn to the opposite side
3 points: Tip to the opposite side
4 points: inability to walk spontaneously, loss of consciousness

Pathological results

HE staining: the tissue morphology of the bleeding site was detected.
Prussian blue staining: determine bleeding in the molding area;

The expression of NeuN (neuron marker), GFAP (astrocyte marker) and Iba1 (microglia marker) in brain tissues were detected by immunohistofluorescence staining.

Apoptosis detection: Tunel fluorescence staining was used to detect the apoptosis of brain tissue.

Cytokines level

Statistical analysis

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison , P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

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