Mouse Model for Endometriosis (EM) Mus musculus (Mouse) Disease model

Endometriosis uterina

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Overview
Properties
  • Prototype SpeciesHuman
  • SourceTransplantation of allograft uterine endometrium induced
  • Model Animal StrainsBalb/c Mice, healthy, female, 6-8W, body weight 20g-22g
  • Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group and Test drug group (three doses)
  • Modeling Period2W
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  • Mouse Model for Endometriosis (EM) Packages (Simulation)
  • Mouse Model for Endometriosis (EM) Packages (Simulation)
  • DSI543Mu01.png Fig. Lesions in operation group mice on 7th days after modeling
  • Mouse Model for Endometriosis (EM) Fig. Lesions in operation group mice on 28d after modeling
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Modeling Method

1. Randomly select one mouse as a donation, killed by dislocation of cervical vertebra, fixed on the operating table, abdominal alcohol disinfection, along the midline ripped abdomen. Find the Y-shaped uterus, carefully pull respectively the left and right uterine, careful separation of bilateral uterine membrane.
2. Breaking away from uterine horns and ovary, the Y-shaped uterus are cut at the left and right uterine bifurcation, into a bowl with physiological saline aseptic curved plate, repeated rinsing the uterine, and separation remaining adhered the mesometrium. Placed two uterine horns in two sterile curved plate, and cut into pieces of 1 * 1mm, and suspended in normal saline.
3.1/2 uterus from one donated mouse is planted in one receptor mouse,and the tissue fragments are put in the saline suspension.
4.Randomly select one mouse as recipients, the left lower quadrant of the mouse umbilical cord was taken as the point of needle insertion., the uterine tissue fragments are injected into recipients through intraperitoneal injection.

Model evaluation

1. Observation: seventh days after modeling, the mice are sacrificed to observe the lesion, and the survived lesions show a variety of forms, such as red, blue, purple, white, orange and other vesicles, and some of the surrounding adhesion.

Pathological results

Histological changes of ectopic endometrium after modeling
The glands and stroma cells are abundant, and the structure of the gland is normal and the blood vessels were abundant.

Cytokines level

Statistical analysis

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison , P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

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