Mouse Model for Acute Posthemorrhagic Anemia (APC) Mus musculus (Mouse) Disease model

Blood loss anemia; Posthemorrhagic anemia

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Overview
Properties
  • Prototype SpeciesHuman
  • SourceInduced by bloodletting operation
  • Model Animal StrainsBalb/c Mice(SPF), healthy, male, age: 4~6weeks, body weight:18g~20g.
  • Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group and Test drug group.
  • Modeling Period4-6 weeks
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  • Mouse Model for Acute Posthemorrhagic Anemia (APC) Packages (Simulation)
  • Mouse Model for Acute Posthemorrhagic Anemia (APC) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Modeling Method

"Orbital vein bloodletting method: after fixing the mouse, use sterilized capillary suction pipe, from the medial canthus by nasal lateral orbital in parallel to the larynx direction after the light pressure to the orbital venous plexus, blood will get into the tube. after bloodletting 6 ~ 8 (0.5 ml), pull out the blood vessels, use sterile gauze or cotton ball to press the eyeball to stop the bleeding. Bloodletting one time the other day for 5 times, can form chronic blood loss anemia model. 24 h after loss of blood the model of acute blood loss anemia will be formed. "

Model evaluation

Pathological results

Cytokines level

Statistical analysis

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±s), using t test and single factor analysis of variance for group comparison , P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

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