Mouse Model for Peripheral Vestibular Disorders (PVD) Mus musculus (Mouse) Disease model

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Overview
Properties
  • Prototype SpeciesHuman
  • SourceInduced by total resection of labyrinth or vestibular nerve section
  • Model Animal StrainsBalb/c Mice(SPF), healthy, male, age: 4~6weeks, body weight:18g~20g
  • Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group and Test drug group.
  • Modeling Period4-6 weeks
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  • Mouse Model for Peripheral Vestibular Disorders (PVD) Packages (Simulation)
  • Mouse Model for Peripheral Vestibular Disorders (PVD) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Modeling Method

Weight about 200g rats and weight about 500g guinea pigs, the examination of the external auditory canal cleaning, tympanic membrane integrity, no spontaneous nystagmus, hearing sharp. Guinea pigs were fixed with halothane, and the chemical method was used to disrupt the side of the labyrinth. The skin of the temporal area was exposed to sterile surgery and exposed to the tympanic membrane. A 0.5 mm hole was drilled with a scalpel. Chloroform into the Chinese ear cavity, but also by tympanic membrane puncture injection. Rats were treated with intramuscular injection of pentobarbital sodium (20mg/kg body weight) and ketamine (60mg/kg body weight). Then, the rats were disrupted by mechanical method. The bone marrow was cut off with bone clamp, Under the microscope, the round window membrane was aseptically cut, exposed to the balloon at the end of the cochlea, and the balloon was sucked with a miniature aspirator, the oval capsule and the ampullary ridge were wound and the wound was wound. Postoperative observation of the behavior of the model. After the end of all the experiments, anesthesia with pentobarbital sodium, the rats and guinea pigs for thoracotomy, exposure to the heart, the left ventricular incision to the aortic intubation, with 0.5% sodium nitrite temperature of saline and dissolved in The 10% formaldehyde solution in the phosphate buffer was perfused and the animals were sacrificed. The temporal specimens were removed and the histopathological changes were observed under microscope. Morphological changes of the vestibular structure were observed under microscope

Model evaluation

Pathological results

Cytokines level

Statistical analysis

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison , P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

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