Mouse Model for Peritoneal Fibrosis (PF) Mus musculus (Mouse) Disease model

PF; Peritoneal Fibrosis

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Overview
Properties
  • Prototype SpeciesHuman
  • SourceIntraperitoneal injection（I.P） of high glucose dialysate
  • Model Animal StrainsKM mice (SPF), healthy, male, 6~8 weeks
  • Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group and Test drug group
  • Modeling Period4w
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  • Mouse Model for Peritoneal Fibrosis (PF) Packages (Simulation)
  • Mouse Model for Peritoneal Fibrosis (PF) Packages (Simulation)
  • DSI819Mu01.jpg Fig. Masson Staining of Peritoneum in Control and Model Group Mice
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Modeling Method

1.Mice were intraperitoneally injected with 4.25% high glucose dialysate (100ml/kg) and LPS (0.1mg/kg) once a day for four weeks.

2.Measure the peritoneal function and take tissue from the parietal peritoneum and visceral peritoneum (mesentery) for relevant indicators.

Model evaluation

1. Observe the changes in peritoneal thickness of experimental animals in each group using Masson staining. Compared with the normal control group, Masson staining showes that the model group had significantly thickened peritoneum, increased extracellular matrix, increased collagen fibers, and significant changes in peritoneal fibrosis.

Pathological results

2. Western blot and Q-PCR detection of fibronectin (FN) and smooth muscle protein（ α- SMA), E. cadherin, expression of type I collagen (Col I).

Cytokines level

Statistical analysis

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison , P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

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