Mouse Model for Pulmonary Fibrosis (PF) Mus musculus (Mouse) Disease model

Lung Fibrosis

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Overview
Properties
  • Prototype SpeciesHuman
  • Sourceinduce by Bleomycin
  • Model Animal StrainsBalb/c Mice(SPF), healthy, male, age: 8~10weeks, body weight:20g~25g.
  • Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group and Test drug group.
  • Modeling Period4-6 weeks
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  • Mouse Model for Pulmonary Fibrosis (PF) Packages (Simulation)
  • Mouse Model for Pulmonary Fibrosis (PF) Packages (Simulation)
  • DSI518Mu01.jpg Fig.MASSON staining for rat lung
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Modeling Method

Modeling method:
SPF C57/BL6 female mice, weight about 18~20g. The model was modeled by tracheal drip bleomycin.
The anesthetized mice were fixed in supine position on the experimental table. After the neck hair was removed, the skin was cut and the trachea was exposed layer by layer. A 1 mL syringe was pierced into the trachea through the gap between the two tracheal cartilage rings and toward the heart, and bleomycin was injected 5mg/kg/L (the control group was injected with the same volume of normal saline) if there was no resistance.

Model evaluation

Development time of pulmonary fibrosis model:On the 7th day after administration, most of the lung tissues showed severe alveolitis, with a large number of neutrophils infiltrating in the alveolar cavity and interstitium, some alveolar cavities destroyed or disappeared, and fibroblasts and capillaries hyperplasia in the lung septum, which were significantly different from normal lung tissues;On the 14th day after administration, pulmonary fibrosis began to form. Macrophages, neutrophils and other inflammatory cells decreased significantly, fibroblasts increased, alveolar septum thickened significantly, collagen deposition; On day 28 after administration, most of the mice developed diffuse pulmonary interstitial fibrosis, in which the pulmonary interstitial was replaced by collagen fibers and fibroblasts, alveolar wall was destroyed, and pulmonary bullae formed, but inflammatory cell infiltration was still visible.

Pathological results

Cytokines level

Statistical analysis

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison , P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

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