Mouse Model for Secretory Otitis Media (SOM) Mus musculus (Mouse) Disease model

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Overview
Properties
  • Prototype SpeciesHuman
  • SourceOVA secretory otitis media
  • Model Animal StrainsBalb/c Mice(SPF), healthy, male, age: 4~6weeks, body weight:18g~20g
  • Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group and Test drug group.
  • Modeling Period4-6 weeks
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  • Mouse Model for Secretory Otitis Media (SOM) Packages (Simulation)
  • Mouse Model for Secretory Otitis Media (SOM) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Modeling Method

BALB/c mice, weight: 25 ~ 30g, age 6 ~ 8 weeks, erythrhea reflect the sensitively, confirmed by earoscopy no external ear canal and middle ear infection. BALB/c mice were given 1.2 mg of ovalbumin (OVA) in 0.6 ml of phosphate buffer (PBS) and intraperitoneally with 5.14 mg of aluminum hydroxide as an adjuvant for 1 week/2 weeks Sensitization; 2 weeks after the dose of 5 ml / kg body weight by intraperitoneal injection of 20% Uralan anesthesia, aseptic technique 1g / L oVA + PBS mixture 15ul before the tympanic membrane into the tympanum, 24 h after the mouse again Anesthesia, observe the tympanic membrane state, and repeated injection 2 times, ear stimulation; caused by secretory otitis media model. The model animals were subjected to otoscopy every day after injection of OVA solution. The mice were sacrificed 2 days after the second challenge. The thickness of the middle mucosa was measured by HE staining and the eosinophils Cells, plasma cells, toluidine blue staining mast cells.

Model evaluation

Pathological results

Cytokines level

Statistical analysis

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison , P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

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