Mouse Model for Tumor Transplantation (TT) Mus musculus (Mouse) Disease model

Transplanted tumors;TE-1;HepG2;HepG2-luc;SK-MES-1;LL/2-luc-M38

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Overview
Properties
  • Prototype SpeciesHuman
  • SourceLiver and lung tumor metastasis caused by melanoma cells (B16)
  • Model Animal StrainsC57BL/6 Mice(SPF), healthy, male, age: 4~6weeks, body weight:18g~20g.
  • Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group and Test drug group(low,medium,high).
  • Modeling Period4-6 weeks
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  • Mouse Model for Tumor Transplantation (TT) Packages (Simulation)
  • Mouse Model for Tumor Transplantation (TT) Packages (Simulation)
  • DSI530Mu03.png Fig. Liver and lung tumor metastasis on mice
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Modeling Method

Mouse tumor formation experiment:
The logarithmic growth cells were taken, digested by trypsin, and then the cells were suspended in the culture medium to adjust the cell density with sterile phosphate buffer solution (PBS).
C57BL/6J mice were selected to establish tumor metastasis model of B16 cells, with 10 mice in each group. In experimental group, 0.1ml of B16 cell suspension at the concentration of 5×10 6 /L was injected into the tail vein. Each group was injected intraperitoneally from the next day after cell inoculation, and then once every other day after 10 days.
On the 21st day, the animals were sacrificed, liver and lung tissues were taken, the number of metastatic nodules was counted under anatomical microscope, digital camera photographed, and the number of lung metastasis nodules in each group of mice was statistically analyzed.

Model evaluation

After the tumor cells were injected into the tail vein of each group, the mice grew well, and there was no significant change in body weight, and no significant difference between groups.On day 21, the mice were sacrificed, and the liver and lung tissues were taken for anatomical observation under microscope. The number of metastatic nodules was counted under anatomical microscope, and the number of liver and lung nodules in the administration group was significantly lower than that in the negative control group.

Take sample：
1.At the end of the experiment, liver tissue, lung tissue and tumor tissue were collected from each group. Each tissue was divided into two parts from the center of the tumor, and one part was fixed in formaldehyde. The other was stored in liquid nitrogen at -80 ° C for molecular biology and WB detection, etc.

Pathological results

Cytokines level

Statistical analysis

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison , P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

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