Mouse Model for Tumor Transplantation (TT) Mus musculus (Mouse) Disease model

Transplanted tumors;TE-1;HepG2;HepG2-luc;SK-MES-1;LL/2-luc-M38

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Overview
Properties
  • Prototype SpeciesHuman
  • SourceInduced by A549-luc intravenous transplantation
  • Model Animal StrainsBalb/c-nude Mice(SPF), healthy, male, age: 4~5weeks, body weight:18g~20g.
  • Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group and Test drug group(low,medium,high).
  • Modeling Period4-6 weeks
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  • Mouse Model for Tumor Transplantation (TT) Packages (Simulation)
  • Mouse Model for Tumor Transplantation (TT) Packages (Simulation)
  • DSI530Mu05.png Fig. Fluorescence in vivo imaging after A549-luc cells were injected in situ for 4w
  • Mouse Model for Tumor Transplantation (TT) Fig. The lung of nude mice injected by A549
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Modeling Method

Cell line: human lung cancer cell line A549-LUC marked with luciferase gene
Tumor formation by tail vein injection in nude mice: Cells with good growth were digested and prepared into cell suspension. After counting, 100μ L (containing 2*106 cells) of cell suspension was taken and inoculated into nude mice through tail vein. Normal diet was carried out every day.
28 days after injection, in vivo imaging was performed.
Procedures for imaging small animals in vivo:
Anesthetized mice.
Fluorescence substrate injection: Sodium fluorescein D was injected intraperitoneally, and 15 mg/mL fluorescein working solution was added at the concentration of 10 uL/g. Injection after 10 minutes, began to fluorescence in vivo imaging.
Open the imaging system of small animals in vivo and set the instrument parameters to select a wavelength range of 500-620nm according to the wavelength of the target protein 530-600nm. Select Fluorescent mode, put the mouse into the imaging camera platform, the software controls the platform to rise and fall to a suitable field of view, automatically turn on the light (bright field) to take the first background image. Next, the light was turned off automatically and the specific photons emitted by the mice were photographed in the dark field without external light. Superposition of bright field and dark field background can intuitively display the location and intensity of specific photons in the animal body to complete the imaging operation. It should be noted that appropriate excitation and emission filters should be selected for fluorescence imaging, while bioluminescence needs to be stimulated by substrate injection before imaging.

Model evaluation

28d live imaging, the lungs were imaged visibly;

Pathological results

Cytokines level

Statistical analysis

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison , P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

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