Rabbit Model for Bladder Obstruction (BO) Oryctolagus cuniculus (Rabbit) Disease model

Bladder Outlet Obstruction

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Overview
Properties
  • Prototype SpeciesHuman
  • Sourceinduced by Ligation of the bladder neck
  • Model Animal StrainsNew Zealand rabbits, healthy, male, weight 2.5-3.0kg.
  • Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group and Test drug group.
  • Modeling Period4-6 weeks
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  • Rabbit Model for Bladder Obstruction (BO) Packages (Simulation)
  • Rabbit Model for Bladder Obstruction (BO) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Modeling Method

Weight 2.5 ~ 3 kg of male New Zealand rabbits, by intravenous according to the dose of 10 mg/kg body weight phenobarbital anesthesia, then fixed on the operating table animals lie on back, the penis insertion F8 catheter, regular disinfection and unhairing lower abdominal surgery.Take a midline incision on the pubic bone, skin incision, separate abs, into the pelvic cavity and the separation of the bladder, bladder neck from blunt trailing edge separation to the contralateral; Silk thread ligation in 1 bladder neck, urethral catheter, close the incision.Animal postoperative day started with gentamicin 10000 U/kg body weight intramuscular 7 d.5 weeks after transvenous with phenobarbital 10 mg/kg body weight, the dose of anesthesia with former method exposed belly, along the urethral catheter insertion F8, via catheter perfusion physiological saline, bladder filling completely.And the catheter around overflow water is defined as a bladder capacity;Then dissect the bladder ligation at first time as a complete resection, suck blood, remove fat after weighing.Open the bladder, ureteral crest above to the bladder at the top of the vertical shape cut out two beams of detrusor full thickness.Muscle bundle of immersion in Tyrode buffer, buffer with 95% oxygen and 5% carbon dioxide equilibrium mixture.The muscle bundle connection at one end to the tension transducer to measure the change of muscle contraction force, input signals through the analog-to-digital conversion system computer for processing.

Model evaluation

Pathological results

Cytokines level

Statistical analysis

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison , P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

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