Rat Model for Alcoholic Hepatitis (AH) Rattus norvegicus (Rat) Disease model

Alcoholic Liver Disease

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Overview
Properties
  • Prototype SpeciesHuman
  • SourceChronic alcoholic liver disease (ALD) induced by long term intake of alcohol
  • Model Animal StrainsSD Rats (SPF), healthy, male, bodyweight:180g~200g
  • Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group and Test drug group (three doses).
  • Modeling Period12 w
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  • Rat Model for Alcoholic Hepatitis (AH) Packages (Simulation)
  • Rat Model for Alcoholic Hepatitis (AH) Packages (Simulation)
  • DSI531Ra01.jpg Fig 1. The liver histopathological changes of rats (HE staining x200)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Modeling Method

1. Rats in normal group were free to drink water.
2. Rats in alcoholic fatty liver disease(AFL) group drink alcohol, the alcohol concentration from the beginning of 5%, each concentration was maintained for a week, in order to 10%, 15%, 20%, 25%, 30%, 35% to 40%, with 40% concentration lasted for 12 weeks.
3. On the course of the experiment, the rats in each group were given ordinary pellet feed for 12 weeks.
4. At the end of the 12th week, the animals were fasted for 12 hours, weighed, and take the blood from the inferior vena cava.

Model evaluation

1. Detection of blood biochemical parameters
The contents of ALT, AST, TC, TG, FFA, LDL-C, HDL-C and blood glucose.

Pathological results

2. Histopathological changes of liver
The results showed that the volume of liver in rats with alcoholic fatty liver and sucrose was significantly increased
Swelling, membrane tension, the edge of the round blunt, milk yellow, greasy section, especially in the abdominal cavity after the accumulation of retroperitoneal fat
Obvious.
HE staining showed that there was no obvious fatty degeneration in the liver of the normal group, and the hepatic lobule structure was normal. In the AFL model group, the liver was filled with a lot of fat degeneration, and the volume of the cells increased.

Cytokines level

The content of TNF-α and ApoB in serum were detected by ELISA method.

Statistical analysis

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison , P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

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