Rat Model for Cystitis Rattus norvegicus (Rat) Disease model

Add to Cart Distributors
Overview
Properties
  • Prototype SpeciesHuman
  • Sourceinduced by Protamine sulfate and Escherichia coli
  • Model Animal StrainsSD Rats(SPF), healthy, male and female, body weight 180g~200g.
  • Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group and Test drug group.
  • Modeling Period2 weeks
Share your citation Upload your experimental result Review Leave a message
Loading...

Share a new citation as an author

Upload your experimental result

Review

Please attach serial No. on instruction manual

Contact us

Please fill in the blank.

Name*
Organization
Address
E-mail address*
Telephone
Inquiry*
Verification code* CheckCode
  • Rat Model for Cystitis Packages (Simulation)
  • Rat Model for Cystitis Packages (Simulation)
  • DSI633Ra01.jpg Picture
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Modeling Method

Model group: rats were intraperitoneally injected with 2% pentobarbital sodium solution. After anesthesia, rats were fixed and urethral opening was used

70% ethanol and iodine tincture were disinfected, and a sterile rigid catheter was inserted into the bladder of rats through the urethra. After the urine was drained, 1 mL protamine sulfate 10 mg/mL was poured and kept for 45 min. After the drug was drained, the urine was washed with phosphate buffer solution (PBS) for 3 times. 1 mL of 750 μg/mL lipopolysaccharide was perfused and kept for 30 min. After the drug was drained, the catheter was rinsed with PBS for 3 times. 24 h later, repeat the operation with the same method. Three days later, the rats were sacrificed, and the bladder tissue and spinal cord tissue were collected.

Control group: The rats were anesthetized by the same method, then a sterile rigid catheter was inserted into the bladder of the rats through the urethra, after the urine was drained, PBS was poured, and the catheter was drained for 30 min. 24 h later, repeat the operation with the same method. Three days later, the rats were sacrificed and the bladder and spinal cord tissues were collected.

Model evaluation

Pathological results

The rats were sacrificed, the bladder was embedded in paraffin and stained with HE. IHC staining indexes: TLR4, NLRP3, CASPASe1

The spinal cord was taken, embedded by OCT, and frozen sections were stained with IHC: TLR4, NLRP3 and CasPASe1.

Degranulated mast cell count: dehydrated bladder tissue, fixed, embedded in paraffin, sliced through toluidine blue blue, dehydrated, transparent, neutral gum sealed sheet. Section white light panoramic scanning, counting degranulated mast cells.

Cytokines level

Statistical analysis

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison , P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

Recommend products