Rat Model for Intervertebral Disc Degeneration (DDD) Rattus norvegicus (Rat) Disease model

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Overview
Properties
  • Prototype SpeciesHuman
  • SourceInduced by surgical method
  • Model Animal StrainsSD rats (SPF class), Male, 6~8W, 200~250g
  • Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group and Test drug group
  • Modeling Period16~20 weeks
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  • Rat Model for Intervertebral Disc Degeneration (DDD) Packages (Simulation)
  • Rat Model for Intervertebral Disc Degeneration (DDD) Packages (Simulation)
  • DSI739Ra01.jpg Fig. X ray image of rats，left：control，right:model
  • Rat Model for Intervertebral Disc Degeneration (DDD) Fig. HE staining of intervertebral disc for model rat
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Modeling Method

After the rats were anesthetized, the posterior median incision was performed,longitudinal incision of the skin and subcutaneous tissue from the atlanto occipital joint to the second thoracic spinous process ranged from 2 to 2.5 cm, blunt free neck muscles to expose the cervical spine sufficiently. From the inside to the outside layer in order to completely cut off 2 ~ 7 cervical interspinous ligament and supraspinous ligament, deep neck atlantoaxial splenius cervicis, longissimus and iliocostalis cervicis and semispinalis muscle, superficial platysma, neck, head and neck trapezius rhomboideus, hemostasis after suture on both sides of sacrospinalis and skin. Continuous 3d injection of penicillin to prevent infection after operation.

Model evaluation

Three months later, all rats were taken anteroposterior and lateral X-ray + lumbar spine

Each segment was dyed with a solid green color (4 segments);

Materials: The whole disc and the upper and lower parts of the vertebral body should also have, the disc unit refers to the disc tissue + upper and lower endplate cartilage + upper and lower vertebral body;

Staining: take the most central part of the disc and make a coronal section (the coronal section is from the ventral side to the dorsal side). Staining should be done on the basis of the entire disc unit. The main observation is the endplate cartilage between the vertebral body and the intervertebral disc.

Pathological results

Cytokines level

Statistical analysis

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison , P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

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