• SPF

Rat Model for Irritable Bowel Syndrome (IBS)

Rats in model group, drug group and pinaverium bromide group were given with 1 g/ml senna decoction by gavage (10 ml/kg), 1 times/day.
The test drug group and pinaverium bromide was crushed and dissolved in distilled water to a concentration of 1.5 mg/ml suspension, 10 ml/kg by gavage, 2 times/day. The normal group and the model group were gavage with the same dose of normal saline for 3 weeks.

1. Abdominal wall retreat reflex (AWR score)
After the model was built, the rats were forbidden to feed water at 24 h before the experiment. After mild anesthesia in rats, the balloon catheter was inserted slowly from the anus, and the 8F catheter (catheter diameter 2.7 mm and maximum balloon volume 3 ml) were inserted from the anus.
The distal end of the balloon is about 1 cm from the anus, and is fixed at the end of the tail of the rat at the outside of the anus at 1cm. Each rat was dilated 3 times with the volume of 1, 1.5, 2 ml, and each expansion lasted 3-5 min, and the interval was 30 s (against intestinal ischemia). Each capacity is repeated 3 times, taking the average.
AWR scoring criteria:
0 points, the rats' mood was basically stable when the colorectal distention was given.
1 points, when given stimulation become unstable, occasionally twisting the head;
2 points, a slight muscle contraction of the abdomen and back abdomen but not lift off the ground;
3, abdomen and back muscles and abdominal contraction lifted off the ground;
4 points, the abdominal muscles contracted strongly, the abdomen was arcuate, and the abdomen and perineum were lifted from the ground.

2. The open field experiment
The box is a high field 30~40cm with a bottom length of 100 cm, and the color of the circumferential wall is black. The open field bottom is divided into 25 4 x 4 cm small squares. The camera is on the top, and the vision covers the whole field. The rats were placed in the middle of the Bay, at the same time and time the camera (5 mins). The computer tracer analysis system is used to analyze the activity state of rats in a certain period of time.
Observation index: through the number of squares (animal limbs from one into another through a lattice), the number of vertical (double forelimbs simultaneously from the animal, or double forelegs on the wall as a number of central upright), time, through the central lattice.

The rat spinal ganglion, the spinal cord and the lumbosacral segment were taken, and the expression and localization of NMDAR1 and NMDAR2B in each group were detected by IHC.

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison , P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.