Rat Model for Acute Pyelonephritis (AP) Rattus norvegicus (Rat) Disease model

Pyelitis; Nephritis

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Overview
Properties
  • Prototype SpeciesHuman
  • Sourceinduced by surgery plus bacterial infections
  • Model Animal StrainsSD Rats(SPF), healthy, male and female, body weight 180g~200g.
  • Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group and Test drug group.
  • Modeling Period4-6 weeks
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  • Rat Model for Acute Pyelonephritis (AP) Packages (Simulation)
  • Rat Model for Acute Pyelonephritis (AP) Packages (Simulation)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Modeling Method

The preparation of e. coli bacteria liquid, will strain inoculation in the broth culture medium, 37 ℃ and 5% CO2 hatch in the 12 h incubation, using gradient plate 106 / ml concentration of bacteria. Weight about 200 g male rats, prohibit drinking water after 18 h through abdominal cavity by 30 mg/kg body weight dose injection of sodium pentobarbital anesthesia. Animals lie on your back plate fixed surgery, regular disinfection abdominal skin and unhairing, sterile surgical towel. Along the lower abdomen for about 2 cm long midline incision, layered cut open abdominal wall into the peritoneal cavity, fully exposed the left ureter and bladder; With sutures 4 from both sides of the middle ureteral respectively to the flank wear needle and elicit sutures, temporarily not ligation, with arterial clip to clip the penis, with TB needle to slow injection of 0.75 ml bacteria liquid inside bladder. Then tighten on both ends of the suture ligation ureter. Layered suture incision, loosen the artery clamp, conventional recovery of animal breeding. 20 h after the surgery, down outside the abdominal wall of ureteral ligation line, make its reopening, respectively with clamp of the arteries in the stipulation time after rat penis, with ether anesthesia, on the operating table, sterile under the condition of open, open, take out blood and urine, cut out around the kidney and spleen, for visual inspection, bacterial cultivation and histologic examination.

Model evaluation

Pathological results

Cytokines level

Statistical analysis

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison , P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

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