Rat Model for Osteoarthritis (OA) Rattus norvegicus (Rat) Disease model

Ostarthritis; Ostearthritis; OA

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Overview
Properties
  • Prototype SpeciesHuman
  • SourceInduced by Iodoacetic acid
  • Model Animal StrainsSD Rats(SPF), healthy, male, 6~8W, body weight 180g~200g.
  • Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group and three Test drug group.
  • Modeling Period4-6 weeks
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  • Rat Model for Osteoarthritis (OA) Packages (Simulation)
  • Rat Model for Osteoarthritis (OA) Packages (Simulation)
  • DSI587Ra05.jpg Fig. eVon Frey for Mechanical allodynia test
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Modeling Method

MIA modeling method: SD rats were fed in SPF environment, adaptive feeding for 7 days, and randomly grouped. Rats in model group and drug group were injected 0.2ml of iodoacetic acid into the joint cavity of rats. The control group was injected with normal saline into the joint cavity.

Model evaluation

Follow-up tests:
　　1.Activity status of SD rats was observed and body weight was recorded;
　　2.Mechanical Allodynia test;
Rats were placed on a metal mesh frame to adapt to the environment, and electron Von Frey wire was used to stimulate the skin surface of the rear claw vertically below, and the minimum force required to induce positive claw retraction was recorded as the claw retraction threshold. The pressure of foot contraction (g) was recorded and measured 4 times within a 2-minute interval. The median value obtained after excluding the maximum and minimum values was used as the pain threshold (g).
　　3. The width of the left knee joint was measured, and the surface conditions of cartilage gloss and color were observed for the separated synovium.

Pathological results

Pathological staining: the knee cartilage was decalcified, embedded in paraffin and sectioned. Then HE, Masson and Safranin-fixed green staining were performed to observe the structural changes of cartilage, and the pathological analysis and statistics were performed.

Cytokines level

Cytokines detection:
　　　Cytokines in arthritis: Tumor necrosis factor α(TNF-α), interleukin 1β(IL-1β) and matrix metalloproteinase-3 (MMP-3) in the articular fluid of rats were detected by ELISA.
　　　Serum cytokines: IL-4, IL-10, IL-1β, TNF-α levels.
Biochemical test: Superoxide dismutase (SOD) and malondialdehyde (MDA) were detected in serum.

Statistical analysis

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison , P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

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