Rat Model for Retinal Ischemia Reperfusion Injury (RIRI) Rattus norvegicus (Rat) Disease model

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Overview
Properties
  • Prototype SpeciesHuman
  • SourceAnterior chamber pressure perfusion
  • Model Animal StrainsSD Rats(SPF), healthy, male, 6~8W ,body weight 180g~200g.
  • Modeling Grouping1.Randomly divided into six group: Control group, Model group, Positive drug group and Tested drug group.
  • Modeling Period1w
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  • Rat Model for Retinal Ischemia Reperfusion Injury (RIRI) Packages (Simulation)
  • Rat Model for Retinal Ischemia Reperfusion Injury (RIRI) Packages (Simulation)
  • DSI846Ra01.jpg Fig.The operation of Anterior chamber pressure perfusion
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Modeling Method

1. Prepare for anesthesia: accurately weighing the weight of the rats and intraperitoneal injection of anesthesia rats;
2. The rats were placed on the operating table, and the right eye of the rats was disinfected with active iodine solution, and kept warm at 37℃.
3. Apply the eye anesthetic evenly to the eyeball.When there was no response that the cotton swab was touched to the eyeball of the rats, the needle of disposable intravenous infusion device was inserted into the middle and back vitreous from the sclera to stop the needle, and the eyeball was observed to change from red to colorless by sliding the flow regulator to the appropriate flow.Retinal ischemia began at this point, and the needle was removed 45 minutes later.During ischemia, care should be taken to stabilize the needle and continue anesthesia to prevent the needle from falling out or moving.

Model evaluation

After 72h of modeling, the animals were sacrificed, the retinal morphology was observed by HE staining, and the changes of Bcl-2 protein and Bax protein in retinal cells were detected by immunohistochemistry.
Results :HE staining showed that the retinal structure of normal control rats was clear and the cells of each layer were closely arranged. In the ischemia-reperfusion group, the retina is highly edematous with loss and disordered distribution of ganglion cells. A large number of Bcl-2 positive cells were observed in the retina of the normal control group, but decreased significantly in the ischemia-reperfusion group.

Pathological results

Cytokines level

Statistical analysis

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison , P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

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