Rat Model for Tourette Syndrome (TS) Rattus norvegicus (Rat) Disease model

Tourette's

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Overview
Properties
  • Prototype SpeciesHuman
  • SourceInduced by 3,3 '-iminodipropionitrile(IDPN)
  • Model Animal StrainsSD Rats(SPF), healthy, male, bodyweight:180g~200g
  • Modeling GroupingRandomly divided into six group: Control group, Model group, Positive drug group (Tiapride Hydrochloride )and Test drug group (three doses) n=15.
  • Modeling Period7d
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  • Rat Model for Tourette Syndrome (TS) Packages (Simulation)
  • Rat Model for Tourette Syndrome (TS) Packages (Simulation)
  • DSI550Ra01.jpg Fig. Trajectory map of the Water Maze Test
  • Rat Model for Tourette Syndrome (TS) Fig. Trajectory map of the Open Field Test
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Modeling Method

1. The rats were injected intraperitoneally with  3,3 '-iminodipropionitrile(IDPN), and the dose: 300 mg/(kg.d) for continuous administration for 7 days.
2. After modeling, the rats were given intragastric administration at the same time every morning. The blank group and model group were given normal saline. The intragastric dose was 1mL/100g.

Model evaluation

Behavioral experiment evaluation criteria
1. Motion behavior assessment standard:
0 points: quiet or normal activity;
1 points: over excited;
2 points: increase the behavior of inquiry, non continuous nasal suction;
3 points: keep running;
4 points: stop running with startle.
2. Stereotyped behavior assessment standard: according to the Diamond score
0 points: no stereotyped movement;
1 points: the body rotation behavior (rotation clockwise or counterclockwise);
2 points: head and neck movement up and down too much (an abnormal movement of head and neck and vertical direction);
3 points: head and neck movement up and down too much and rotating motion;
4 points: head to the side of the pendulum, with head and neck movement up and down too much.

Behavioral assessment time: the behavioral experiment was performed every other day for 7 days after modeling, and a total of 5 behavioral tests were performed.

Pathological results

Cytokines level

Dopamine(DA), Dopamine receptor 2(D2R):
After the last administration of fasting but free drinking water at night, 5 rats in each group were randomly selected, after the end of the blood into the EDTA anticoagulant tube, frozen centrifugation (3000 rpm, 10 minutes), take the supernatant stored at -80℃.
ELISA kit was used to detect the content of DA and D2R in serum and striatum

Statistical analysis

SPSS software is used for statistical analysis, measurement data to mean ± standard deviation (x ±ｓ), using t test and single factor analysis of variance for group comparison , P<0.05 indicates there was a significant difference, P<0.01 indicates there are very significant differences.

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