• KO-Validated
Polyclonal Antibody to Prostaglandin Endoperoxide Synthase 2 (PTGS2) Homo sapiens (Human) Polyclonal antibody

PGG/HS; PGHS2; PHS2; HCox2; COX2; Cyclooxygenase 2; Prostaglandin G/H Synthase And Cyclooxygenase; Prostaglandin H2 synthase 2

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Overview
Properties
  • SourcePolyclonal antibody preparation, Host Rabbit
  • Ig Type IgG, Potency n/a
  • PurificationAntigen-specific affinity chromatography followed by Protein A affinity chromatography
  • LabelNone
  • Immunogen RPA699Hu01-Recombinant Prostaglandin Endoperoxide Synthase 2 (PTGS2)
  • Buffer Formulation0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
  • TraitsLiquid, Concentration 500µg/mL
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  • Polyclonal Antibody to Prostaglandin Endoperoxide Synthase 2 (PTGS2) Packages (Simulation)
  • Polyclonal Antibody to Prostaglandin Endoperoxide Synthase 2 (PTGS2) Packages (Simulation)
  • Polyclonal Antibody to Prostaglandin Endoperoxide Synthase 2 (PTGS2) Figure. DAB staining on IHC-P; Samples: Human Liver Tissue.
  • Polyclonal Antibody to Prostaglandin Endoperoxide Synthase 2 (PTGS2) Figure. DAB staining on IHC-P;
    Samples: Human Breast cancer Tissue.
  • Polyclonal Antibody to Prostaglandin Endoperoxide Synthase 2 (PTGS2) Figure. DAB staining on IHC-P;
    Samples: Human Pancreas Cancer Tissue.
  • Polyclonal Antibody to Prostaglandin Endoperoxide Synthase 2 (PTGS2) Figure. DAB staining on IHC-P;
    Samples: Human Kidney Tissue.
  • PAA699Hu01.jpg Figure. Western Blot; Sample: Recombinant PTGS2, Human.
  • Polyclonal Antibody to Prostaglandin Endoperoxide Synthase 2 (PTGS2) Figure. Western Blot; Sample: Mouse RAW 264.7 Cells.
  • Polyclonal Antibody to Prostaglandin Endoperoxide Synthase 2 (PTGS2) Knockout Varification:
    Lane 1: Wild-type RAW264.7 cell lysate;
    Lane 2: PTGS2 knockout RAW264.7 cell lysate;
    Predicted MW: 69kd
    Observed MW: 69kd
    Primary Ab: 5µg/ml Rabbit Anti-Human PTGS2 Antibody
    Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
    (Catalog: SAA544Rb19)
  • Certificate ISO9001: 2008, ISO13485: 2003 Registered

Specifity

The antibody is a rabbit polyclonal antibody raised against PTGS2. It has been selected for its ability to recognize PTGS2 in immunohistochemical staining and western blotting.

Usage

Western blotting: 0.5-2µg/mL
Immunohistochemistry: 5-20µg/mL
Immunocytochemistry: 5-20µg/mL
Optimal working dilutions must be determined by end user.

Storage

Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Stability

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Organism Species More: Mus musculus (Mouse)

Citations

  • Diallyl sulfide protects against ultraviolet B-induced skin cancers in SKH-1 hairless mouse: analysis of early molecular events in carcinogenesisWiley: source
  • Molecular mechanisms underlying chemopreventive activities of glycyrrhizic acid against UVB-radiation-induced carcinogenesis in SKH-1 hairless mouse epidermisPubMed: 21545294
  • Curcumin Protects against UVB-Induced Skin Cancers in SKH-1 Hairless Mouse: Analysis of Early Molecular Markers in CarcinogenesisPubMed: 22888366
  • Decalepis hamiltonii inhibits tumor progression and metastasis by regulating the inflammatory mediators and nuclear factor κB subunitsPubmed: 24013642
  • Multitargeted protective effect of Abacopteris penangiana against carrageenan-induced chronic prostatitis in rats.Pubmed: 24211397
  • Relationship between epidermal growth factor receptor (EGFR) mutation and serum cyclooxygenase-2 Level, and the synergistic effect of celecoxib and gefitinib on EGFR expression in non-small cell lung cancer cellsPubMed: 26464643
  • Monitoring of cyclooxygenase-2 levels can predict EGFR mutations and the efficacy of EGFR-TKI in patients with lung adenocarcinomaPubMed: 26191267

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